From the fifth day of larval transfer to the day before hatching, i.e. the tenth day, the queen cells can complete their development cycle in an incubator.
The use of incubators is more and more widespread among Italian bee-keepers: they group cells 6 days after the larval transfer and have the queens born in an incubator. Since the Italian weather is highly variable, this is an intelligent method which allows the identification of virgin bees before putting them inside the nuclei. This insertion must be performed within four days after the birth. It is also necessary to know that bees better accept a queen cell ready to hatch than a virgin queen bee.